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Badania układu hemostazy – praktyczne aspekty - Vetkompleksowo – serwis dla lekarzy weterynarii
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Wyszukaj w serwisie


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Badania układu hemostazy – praktyczne aspekty


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Błędy przedlaboratoryjne

Termin „błędy przedlaboratoryjne” odnosi się do wszystkich procedur mających miejsce przed wykonaniem docelowego badania. Ich nieodpowiednie przeprowadzenie może stanowić najważniejsze źródło błędnych lub niemożliwych do interpretacji wyników, co w następstwie wiąże się z koniecznością powtarzania badań i/lub rozszerzenia diagnostyki. Dokładne zapoznanie się ze wszystkimi czynnikami wpływającymi na powstawanie błędów przedlaboratoryjnych pomoże lekarzom zmniejszyć ryzyko ich powstawania.

hemostazy
Tab. 1. Czynniki wpływające na powstanie hemolizy i skrzepu w próbce krwi z cytrynianem sodu 3,2%

Odpowiednie przygotowanie pacjenta

Pierwszym etapem odpowiedniej preanalityki jest prawidłowe przygotowanie pacjenta do pobrania krwi. Zwierzę powinno być na czczo, po minimum 8-godzinnej (najlepiej 12-godzinnej) głodówce, aby uniknąć lipemii w osoczu (24). Jest to niezwykle istotne ze względu na metodę pomiaru wykorzystywaną do oznaczenia czasów krzepnięcia. Badania te są zazwyczaj wykonywane na automatycznych analizatorach koagulologicznych wykorzystujących metodę chronometryczną – opierającą się na pomiarze czasu zmiany gęstości optycznej zachodzącej podczas reakcji wykrzepiania, oraz chromogenną – bazującą na reakcji kolorymetrycznej, w której określana jest szybkość zmian absorbancji materiału próbki (5). Dlatego każda zmiana zabarwienia osocza (lipemia, hemoliza, ikteria) może wpłynąć na zafałszowanie wyników.

Dzień przed pobraniem krwi do badań należy ograniczyć spożycie posiłków o wysokiej zawartości tłuszczu, aby uniknąć tworzenia się chylomikronów w surowicy, które mogą zakłócić transmisję światła w analizatorze [...]

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Mogą zainteresować Cię również


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Wizytówka firmy


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Sklep


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Poznaj nasze serwisy